WGS data set of HEK293 cells stably expressing BEACON2 base editing system

BEACON2 expression cassette was stably integrated into HEK293 cells. Cells were transfected with either non targeting gRNA or a 16 gRNA array targeting 6 different genes. WGS was performed on 3 clones edited with the 16 gRNA array, and one non targeting gRNA clone to analyze off-target effects.将BEACON2表达盒（BEACON2 expression cassette）稳定整合至HEK293细胞内。随后将细胞分为两组，分别采用非靶向向导RNA（non-targeting gRNA）与靶向6个不同基因的16条向导RNA阵列（16 gRNA array）进行转染。对3组经16条向导RNA阵列编辑的单克隆细胞以及1组经非靶向向导RNA编辑的单克隆细胞开展全基因组测序（Whole Genome Sequencing, WGS），以分析脱靶效应。