COI metabarcoding data from arthropod pollinator communities in burned and unburned sites of California

Novel wildfire regimes are rapidly changing global ecosystems and pose significant challenges for biodiversity conservation and ecosystem management. In this study, we used DNA metabarcoding to assess the response of arthropod pollinator communities to large-scale wildfires across diverse habitat types in California. We sampled six reserves within the University of California Natural Reserve System (UCNRS), each of which was partially burned in the 2020 Lightning Complex wildfires in California. Using yellow pan traps to target pollinators, we collected arthropods from burned and unburned sites across multiple habitat types including oak woodland, redwood, scrub, chamise, grassland, forest, and serpentine habitats. We found no significant difference in alpha diversity values between burned and unburned sites; instead, seasonal variations played a significant role in arthropod community dynamics, with the emergence of plant species in Spring promoting increased pollinator richness at all..., Sampling in the University of California Reserve System (UCNRS)
The UC Natural Reserve System comprises 47,000 acres distributed across most of the major California ecosystems. Between August 15-16, 2020, an exceptionally fierce electrical storm crossed northern California, peaking at 200 lightning strikes in 30 minutes. Over the following 72–96 hours, more than 12,000 lightning strikes sparked multiple wildfires around the Greater Bay Area. Many of the smaller fires merged into four large fire complexes, including two in Monterey County. Three of these fire complexes are considered to be the second, third, and fourth largest fires in California history (CAL Fire, 2022). These fires burned eight of the UC Natural Reserve System’s 41 reserves and consumed more than 20,000 acres of reserve lands. Affected NRS reserves include Año Nuevo Island Reserve (CZU Lightning Complex), Blue Oak Ranch Reserve (SCU Lightning Complex), Landels-Hill Big Creek Reserve (Dolan Fire), Hastings Natural Histo..., , # COI metabarcoding of arthropod pollinator communities in burned and unburned sites of California

## Description of the data and file structure

Data includes:

**a)** **mco_fire_all.csv** - Output sequences from DADA2 without any additional filtering. Columns include: the sequence (*seq*), the number of reads associated with the sequence (*asv_size)*, an ASV identifier (*asv*), the sample in which the sequence was detected (*sample*), the number of reads detected for a particular sample (*count*), the marker (*marker*), year collected (*year*), which PCR replicate the specific sequence was from (*rep*), the UC reserve (*reserve*), the burn status (*status*) and the site identifier (*site*). Reserves are abbreviated as follows - HT: Hastings, QR: Quail Ridge, AN: Ano Nuevo, BO: Blue Oak, ML: McLaughlin and BR: Big Creek.

**b)** **mco_97_wsites.csv** - Sequence data after additional curation and clustering at 97% and the associated site data. Columns include the ASV identifier (*asv...

新型野火动态正快速改变全球生态系统，并给生物多样性保护与生态系统管理带来严峻挑战。本研究采用DNA元条形码（DNA metabarcoding）技术，评估了加利福尼亚州不同生境类型下，节肢动物传粉者群落（arthropod pollinator communities）对大规模野火的响应。本研究在加州大学自然保护区系统（University of California Natural Reserve System, UCNRS）内的6处保护区开展采样，所有保护区均在2020年加州闪电复合体野火中遭受部分过火。本研究采用黄色盘形诱捕器（yellow pan traps）靶向采集传粉者，从橡树林、红杉林、灌丛、查米塞灌丛、草原、森林以及蛇纹岩土生境等多种生境的过火与未过火样点中收集节肢动物。本研究未发现过火与未过火样点的α多样性（alpha diversity）存在显著差异；相反，季节波动对节肢动物群落动态具有显著影响，春季植物萌发可提升所有样点的传粉者物种丰富度……

# 加州大学保护区系统（UCNRS）采样说明

加州大学自然保护区系统总面积达4.7万英亩，覆盖加州绝大多数主要生态系统类型。2020年8月15至16日，一场强度极高的雷暴过境北加州，峰值时段30分钟内累计闪电达200次。随后72至96小时内，超过1.2万次闪电在大旧金山湾区周边引发多起野火。多数小型野火最终合并为4处大型火复合体，其中2处位于蒙特雷县。其中3处火复合体被列为加州历史上第二、第三、第四大规模野火（CAL Fire, 2022）。此次野火烧毁了加州大学自然保护区系统41处保护区中的8处，过火保护区土地面积超过2万英亩。受影响的保护区包括阿尼奥新诺岛保护区（CZU闪电复合体野火）、蓝橡树牧场保护区（SCU闪电复合体野火）、兰代尔斯-希尔大溪保护区（多兰野火）、黑斯廷斯自然历史……

# 基于COI元条形码的加州过火与未过火样地节肢动物传粉者群落研究

## 数据与文件结构说明

本数据集包含以下文件：
**a)** **mco_fire_all.csv**：未经额外过滤的DADA2算法输出序列。列字段包括：序列（seq）、序列关联读长数（asv_size）、扩增子序列变异体标识符（amplicon sequence variant, ASV）、序列检出样本（sample）、单样本检出读长数（count）、标记基因（marker）、采样年份（year）、特定序列所属PCR重复组（rep）、加州大学保护区名称（reserve）、火烧状态（status）以及样点标识符（site）。保护区缩写对应关系如下：HT=黑斯廷斯（Hastings）、QR=鹌鹑岭（Quail Ridge）、AN=阿尼奥新诺（Ano Nuevo）、BO=蓝橡树（Blue Oak）、ML=麦克劳克林（McLaughlin）、BR=大溪（Big Creek）。
**b)** **mco_97_wsites.csv**：经过额外整理并以97%相似度聚类后的序列数据及关联样点信息，列字段包括ASV标识符（asv……