Supercolonization of larval and juvenile zebrafish with bacterial strains isolated from zebrafish guts.

We selected several bacterial genera for which the relative abundance was altered in response to TBT-Cl exposure in Experiment 1, and of which we had culturable bacterial isolates from zebrafish intestines. We exposed conventionally-raised zebrafish raised to suspensions containing one of these bacterial strains for 14 days. Each bacterial treatment had three tank replicates. We sampled five guts as well as 150 ml of rearing water per tank at the end of the bacterial exposure.

我们选取了在实验1中经三丁基氯化锡（TBT-Cl）暴露后相对丰度发生改变的若干细菌属，且已从斑马鱼肠道中获得这些菌属的可培养细菌分离株。我们将常规饲养的斑马鱼暴露于含有上述其中一种细菌菌株的悬浮液中，暴露时长为14天。每个细菌处理组均设置3个水族箱重复组。在细菌暴露结束后，我们从每个水族箱中采集5份肠道样本以及150毫升养殖用水。