Blood proteome profiling using aptamer-based technology for rejection biomarker discovery in transplantation

In this study, blood proteome characterization in face transplantation using longitudinal serum samples from six face transplant patients was carried out with SOMAscan platform. Overall, 24 serum samples from 13 no-rejection, 5 nonsevere rejection and 6 severe rejection episodes were analyzed.Files attached:HMS-16-007.20160218.adat - raw SomaScan dataset presented in adat format.HMS-16-007_SQS_20160218.pdf - technical validation report on the dataset.HMS-16-007.HybNorm.20160218.adat - SomaScan dataset after hybridization control normalization presented in adat format.HMS-16-007.HybNorm.MedNorm.20160218.adat - SomaScan dataset after hybridization control normalization and median signal normalization presented in adat format.HMS-16-007.HybNorm.MedNorm.Cal.20160218.adat - SomaScan dataset after hybridization control normalization, median signal normalization, and calibration presented in adat format.HMS-16-007.HybNorm.MedNorm.Cal.20160218.xls - SomaScan dataset after hybridization control normalization, median signal normalization, and calibration presented in Microsoft Excel Spreadsheet format.Patients_metadata.txt – metadata file containing patients’ demographic and clinical information presented in tab-delimited text format. Metadata is linked to records in the SomaScan dataset via ‘SampleType’ column.SciData_R_script.R – this script is given as an example of a downstream statistical analysis of the HMS-16-007.HybNorm.MedNorm.Cal.20160218.adat dataset.SciData_R_script_SessionInfo - Session information for SciData_R_script.R script.本研究针对面部移植领域，采集6名面部移植患者的纵向血清样本，采用SOMAscan平台开展血液蛋白质组表征分析。最终共分析了13例无排斥反应、5例非重度排斥反应及6例重度排斥反应事件对应的24份血清样本。 本次公开的数据集附件如下： 1. HMS-16-007.20160218.adat：adat格式的原始SomaScan数据集 2. HMS-16-007_SQS_20160218.pdf：该数据集的技术验证报告 3. HMS-16-007.HybNorm.20160218.adat：经杂交对照标准化处理后的adat格式SomaScan数据集 4. HMS-16-007.HybNorm.MedNorm.20160218.adat：经杂交对照标准化与中位数信号标准化处理后的adat格式SomaScan数据集 5. HMS-16-007.HybNorm.MedNorm.Cal.20160218.adat：经杂交对照标准化、中位数信号标准化及校准处理后的adat格式SomaScan数据集 6. HMS-16-007.HybNorm.MedNorm.Cal.20160218.xls：经杂交对照标准化、中位数信号标准化及校准处理后的Microsoft Excel电子表格格式SomaScan数据集 7. Patients_metadata.txt：以制表符分隔的文本格式患者元数据文件，包含患者人口统计学与临床信息，该元数据可通过‘SampleType’列与SomaScan数据集中的记录建立关联 8. SciData_R_script.R：用于演示对HMS-16-007.HybNorm.MedNorm.Cal.20160218.adat数据集开展下游统计分析的R脚本 9. SciData_R_script_SessionInfo：SciData_R_script.R脚本的运行环境会话信息