RNA-seq of P. vivax parasite populations, enriched for cytoadhesion and rosetting phenotypes, from vivax malaria patients with scarce parasitemia from the low transmission Brazilian Amazonian endemic region. RNA-seq of P. vivax parasite populations, enriched for cytoadhesion and rosetting phenotypes, from vivax malaria patients with scarce parasitemia from the low transmission Brazilian Amazonian endemic region

Deep sequencing of the transcriptome of P. vivax parasite populations from vivax malaria patients with scarce parasitemia from the low transmission Brazilian Amazonian endemic region we the aim of better understanding the molecular mechanisms behind this cytoadherence and rosetting phenotypes by identifying proteins, especially parasitic ligands, which might be important for the P. vivax adhesion capacity within the human host. We used RNA-seq coupled with parasite enrichment from field samples and cytoadherence and rosetting assays to privilege the sequence of the whole transcriptome of parasite populations with distinct adhesive characteristics and, also assess the human host immune-related expression profile in the context of vivax malaria disease.

本研究对巴西亚马逊低传播疟疾流行区中原虫血症水平较低的间日疟原虫（Plasmodium vivax, P. vivax）感染患者体内的疟原虫群体转录组开展深度测序，旨在通过鉴定可能对间日疟原虫在人体宿主内黏附能力至关重要的蛋白质（尤其是寄生虫配体），深入解析该病原体细胞黏附与玫瑰花结形成表型背后的分子机制。本研究结合临床现场样本的寄生虫富集技术、细胞黏附与玫瑰花结形成实验，采用RNA测序（RNA-seq）技术优先获取具有不同黏附特性的疟原虫群体的完整转录组序列，并同时评估间日疟疾病背景下人体宿主的免疫相关表达谱。