Tracking Early Mammalian Organogenesis – Prediction and Validation of Differentiation Trajectories at Whole Organism Scale

This project is based on the extension of the single cell transcriptomics atlas of mouse development towards E9.5. Inference of cell differentiation trajectories was carried out using Waddington-Optimal Transport, and the resulting computational reconstruction was contrasted with grafting experiments as well as complementary scRNA-seq experiments.

本项目基于小鼠发育至E9.5阶段的单细胞转录组图谱（single cell transcriptomics atlas）开展拓展研究，采用沃丁顿最优传输（Waddington-Optimal Transport）推断细胞分化轨迹，并将所得计算重构结果与移植实验及互补单细胞RNA测序（scRNA-seq）实验结果进行对比分析。