Coastal marshes (Louisiana) Targeted Locus (Loci)

This study was done to evaluate changes in indigenous microbial communities from coastal marsh sediments after the Gulf of Mexico oil spill in 2010. This study was conducted from eleven coastal marshes in southern Louisiana. Samples were acquired from May 2010 through August 2013, focusing on sediments approximately 1 m offshore from the edge of the marsh. Marshes were oiled from late June-August 2010, and some marshes were reoiled in 2011 from tropical storms and hurricanes. Some marshes close to the Mississippi River were impacted by freshwater diversions in 2010. Bacterial communities were assessed from sediments at 0-1 cm and 1-2 cm depth intervals. The results are amplicon pyrosequence libraries of single-direction reads generated using multiplexed, bar-coded primers targeting the V1-V3 region of the bacterial of 16S rRNA gene.