Klebsiella_pneumoniae_sequencing_Giani_

The collection of isolates that we would like to sequence consists in 163 K. pneumoniae isolates collected in the framework of an Italian survey on carbapenem non-susceptible K. pneumoniae promoted by the Antibiotic-Resistance-Istituto Superiore di Sanità surveillance network. The survey involved 24 different centers distributed across the country. Each center collected all consecutive non-replicate clinical isolates of K. pneumoniae from blood or cerebrospinal fluid (CSF) with MICs >1 mg/L for meropenem and/or imipenem during the period March-July 2016. A preliminary characterization by PCR of showed that the collection is represented by 152 blaKPC 4 blaVIM, 3 blaOXA-48 positive isolates. Sequencing all collected isolates should be useful to: i) obtain data about possible additional mechanisms of resistance, ii) obtain data about the clonality of the KPC-producing isolates; iii) to compare this recent collection with older collections, to obtain new insights about the evolution of the CC258 population.本研究拟开展测序的分离株集合，源自意大利高等卫生研究院（Istituto Superiore di Sanità）抗生素耐药监测网络发起的一项针对碳青霉烯类不敏感肺炎克雷伯菌（Klebsiella pneumoniae, K. pneumoniae）的全国调查项目，共包含163株肺炎克雷伯菌分离株。该调查覆盖全国24家不同的临床研究中心，每家中心在2016年3月至7月期间，收集了所有连续纳入的非重复临床分离株——均为血液或脑脊液（Cerebrospinal Fluid, CSF）样本中分离得到、美罗培南和/或亚胺培南最低抑菌浓度（Minimum Inhibitory Concentration, MIC）＞1mg/L的肺炎克雷伯菌。经聚合酶链式反应（Polymerase Chain Reaction, PCR）对分离株进行初步表征后确认，本分离株集合包含152株blaKPC阳性、4株blaVIM阳性及3株blaOXA-48阳性的菌株。对所有收集的分离株进行测序，将有助于实现以下目标：① 获取潜在额外耐药机制的相关数据；② 明确产KPC酶菌株的克隆特性；③ 将本次新近收集的菌株与既往收集的菌株进行对比分析，以深入解析克隆复合体258（Clonal Complex 258, CC258）种群的演化规律。