Transcriptomic profiling of Linolenic Acid-responsive genes in ROS signalling from RNA-seq data in Arabidopsis

We describe the transcriptional profiles upon 1 mM linolienic acid (Ln) treatment in Arabidopsis cell suspension cultures. Ln is an important fatty acid in plant. We found that Ln treatment induced the expression of 533 genes and repressed the expression of 2501 gene. GO analysis indicated that most of those genes related to Reactive Oxygen Species signaling, abiotic and biotic stress responses, and/or Jasmonic Acid biosynthesis and signaling. This study provides basic information on how Ln regulates the gene expression and fulfils its role in abiotic and biotic stresses.本研究报道了1 mM 亚麻酸（linolienic acid, Ln）处理拟南芥（Arabidopsis）悬浮培养细胞后的转录组表达谱。Ln是植物中一类重要的脂肪酸。本研究发现，Ln处理可诱导533个基因的表达，同时抑制2501个基因的转录。基因本体（Gene Ontology, GO）分析结果显示，上述差异表达基因主要富集于活性氧（Reactive Oxygen Species, ROS）信号转导、非生物与生物胁迫响应，以及茉莉酸（Jasmonic Acid, JA）的生物合成与信号通路。本研究为阐明Ln调控植物基因表达的分子机制，及其在非生物与生物胁迫响应中的功能提供了基础数据支撑。