Ventral hippocampal transcriptome analysis after acute corticosterone exposure.. Ventral hippocampal transcriptome analysis after acute corticosterone exposure.

Aim: We investigated the effect of acute corticosterone exposure 3 hours after subcutaneous injection on the ventral hippocampus transcriptome. Methods: Adult male mice were used in the experiment. Mice were sacrificed 3 hours after corticosterone (3mg/kg) of vehicle injection and the ventral hippocampus was dissected and snap-frozen. Subsequently, total RNA was isolated and send for 100bp paired-end sequencing by BGI. Overall design: Ventral hippocampal mRNA profiles of male mice studying corticosterone effects.

研究目的：本研究旨在探究皮下注射3小时后急性皮质酮暴露对腹侧海马转录组的影响。实验方法：本实验采用成年雄性小鼠。在向小鼠注射皮质酮（3mg/kg）或赋形剂3小时后处死动物，解剖获取腹侧海马组织并快速冷冻。随后提取总RNA，交由BGI采用100bp双端测序技术完成转录组测序。整体实验设计：本实验以雄性小鼠的腹侧海马mRNA表达谱为研究对象，用于分析皮质酮的相关生物学效应。