Data for: Modulation of the transcriptomic profile of the R2C tumor Leydig cell line by the adipose tissue derived hormone leptin

We examined if leptin could alter the transcriptome of the constitutively steroidogenic rat tumor Leydig cell line R2C. These cells were treated with high levels leptin (1000 ng/ml) for 4 h, followed by mRNA extraction and RNA-Seq analysis. Total RNA extractions from cultured R2C Leydig cells were performed using the E.Z.N.A. Total RNA kit (Omega Bio-Tek, Inc., Norcross, GA). Libraries were constructed using the TruSeq Stranded mRNA LT (Illumina) for cDNA synthesis and polyA enrichment. Quality and quantification of libraries were assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies) and KAPA SYBR® FAST qPCR Kit (KAPA Bio Systems), respectively. Transcriptomic sequencing was performed on an Illumina NextSeq 500 in paired reads layout with approximately 13 million reads per sample and 75 bp of sequencing length. Libraries and next generation sequencing were completed by Applied Biological Materials Inc. (Richmond, BC, CAN). Paired-end raw reads are available in FASTQ format.

本研究探讨瘦素（leptin）是否可改变组成型类固醇生成大鼠睾丸间质细胞瘤细胞系R2C的转录组。将该细胞以高浓度瘦素（1000 ng/ml）处理4小时，随后进行mRNA提取与RNA测序（RNA-Seq）分析。培养的R2C睾丸间质细胞的总RNA提取采用E.Z.N.A.总RNA提取试剂盒（Omega Bio-Tek公司，佐治亚州诺克罗斯市）完成。测序文库构建采用Illumina的TruSeq Stranded mRNA LT试剂盒进行cDNA合成与polyA富集。文库的质量与浓度分别通过安捷伦2100生物分析仪（Agilent Technologies）与KAPA SYBR® FAST定量PCR试剂盒（KAPA Bio Systems）进行评估。转录组测序在Illumina NextSeq 500平台上完成，采用双端测序模式，每个样本约1300万条测序读段，测序读长为75 bp。文库构建与下一代测序均由加拿大不列颠哥伦比亚省里士满市的Applied Biological Materials Inc.完成。双端原始测序读段以FASTQ格式公开可用。