affy_popsec_nancy_leaves2008_poplar -Molecular bases of acclimatation and adaptation to water deficit in poplar

affy_popsec_nancy_leaves_poplar - affy_popsec_nancy_leaves2008_poplar - This project aims to identify genes of interest for water deficit acclimation and/or adaptation in a tree species: poplar. We look for genes and gene expression networks related to drought stress. We intend to analyse the transcriptome in mature leaves, in two genotypes, Carpaccio and Soligo, at various stages and intensities of stress. During water deficit, leaves underwent many processes aiming to maintain cells integrity such as water status adjustment through osmoregulation or cell detoxication. These analyses intend to identify genes of interest involved in homeostasis maintenance. The comparison between medium and severe stress intensities and between early and long term stresses will power the selection of genes of interest. The co-analysis of two genotypes of contrasted tolerance to water deficit should help to discriminate genes presenting a potential adaptative character from genes responding passively to the constraint-In a first experiment, two poplar clones, Soligo (S) and Carpacio (C) were submitted to 4 treatments: control, mild water deficit, moderate water deficit (12-day long for both) and early-drought stress (about 36-h long). Growth and physiology was characterised on a batch of plants and samples collected on another batch of plants. In a second experiment, two poplar clones, Soligo (S) and Carpacio (C) were submitted to 2 treatments: control and moderate water deficit. Mature leaves were collected and total RNAs were extracted from each tree individually. Two pools of 3 (or 2) individuals were made using equimolar ratio. A pool is considered as one biological replicate and corresponds to one Affymetrix slide. Keywords: treated vs untreated comparison 8 arrays - poplar

affy_popsec_nancy_leaves_poplar - affy_popsec_nancy_leaves2008_poplar
本项目旨在鉴定林木（杨树）中参与水分亏缺驯化与/或适应过程的目标基因，挖掘与干旱胁迫相关的基因及基因表达调控网络。我们计划对两个基因型（genotype）的成熟叶片在不同胁迫阶段与胁迫强度下的转录组（transcriptome）进行分析，所用基因型为Carpaccio与Soligo。
在水分亏缺条件下，叶片会启动一系列维持细胞完整性的生理过程，例如通过渗透调节（osmoregulation）维持水分状态、启动细胞解毒作用等。本系列分析旨在鉴定参与内稳态（homeostasis）维持的目标基因。通过对比中等强度与重度干旱胁迫、短期与长期干旱胁迫下的转录组数据，将有助于筛选目标基因。对两个水分亏缺耐受性存在显著差异的基因型进行联合分析，将有助于区分具有潜在适应特性的基因与仅对胁迫产生被动响应的基因。
在第一轮实验中，我们对两个杨树无性系Soligo（S）与Carpacio（C）施加4种处理：对照组、轻度水分亏缺、中度水分亏缺（两种处理均持续12天）以及早期干旱胁迫（持续约36小时）。选取一批植株进行生长与生理特性表征，另一批植株用于采集实验样本。
在第二轮实验中，我们对两个杨树无性系Soligo（S）与Carpacio（C）施加2种处理：对照组与中度水分亏缺处理。采集成熟叶片，并从每棵植株中单独提取总RNA。以等摩尔比将3（或2）个个体的样本混合，构建2个混合样本池。每个混合样本池视为一个生物学重复，对应一张Affymetrix芯片。
关键词：处理组与对照组对比；8张芯片；杨树