Table_1_Tissue-specific metabolomic profiling after cardiopulmonary bypass in fetal sheep.XLSX

ObjectiveFetal cardiopulmonary bypass (CPB) is essential to fetal heart surgery, while its development is limited by vital organ dysfunction after CPB. Studying organ metabolism may help to solve this problem. The objective of this study was to describe the tissue-specific metabolic fingerprints of fetal sheep under CPB and to associate them with organ functions.
MethodsTen pregnant ewes at 90–120 days of gestation were randomly divided into two groups. The bypass group underwent a 1-h fetal CPB, whereas the control group underwent only a fetal sternotomy. During bypass, echocardiography, blood gases, and blood biochemistry were measured. After bypass, lambs were sacrificed, and tissues of the heart, liver, brain, kidney, and placenta were harvested. The metabolites extracted from these tissues were analyzed using non-targeted metabolomics based on liquid chromatography-mass spectrometry techniques.
ResultsAll tissues except the placenta displayed significant metabolic changes, and the fetal heart displayed obvious functional changes. Fetal sheep that underwent CPB had common and tissue-specific metabolic signatures. These changes can be attributed to dysregulated lipid metabolism, altered amino acid metabolism, and the accumulation of plasticizer metabolism.
ConclusionFetal CPB causes tissue-specific metabolic changes in fetal sheep. Studying these metabolic changes, especially cardiac metabolism, is of great significance for the study of fetal CPB.

研究目的：胎儿体外循环（cardiopulmonary bypass, CPB）是胎儿心脏手术的核心手段，但其临床转化受到术后重要器官功能障碍的制约。探究器官代谢特征或可为破解该瓶颈提供有效路径。本研究旨在描述体外循环环境下胎羊的组织特异性代谢指纹，并建立其与器官功能的关联。
方法：选取10只妊娠90~120天的妊娠母羊，随机分为两组。体外循环组实施1小时胎羊体外循环，对照组仅实施胎儿胸骨切开术。体外循环期间，同步开展超声心动图检测、血气分析及血液生化指标测定。体外循环结束后，处死胎羊并采集心脏、肝脏、脑组织、肾脏及胎盘组织。采用基于液相色谱-质谱联用技术的非靶向代谢组学方法，对上述组织提取的代谢物进行定性定量分析。
结果：除胎盘组织外，其余所有受试组织均出现显著代谢改变，胎儿心脏亦表现出明确的功能异常。接受体外循环的胎羊存在共通性与组织特异性兼具的代谢特征，此类改变可归结为脂质代谢失调、氨基酸代谢紊乱及增塑剂代谢产物蓄积。
结论：胎儿体外循环可诱导胎羊产生组织特异性代谢变化。对这类代谢改变，尤其是心脏代谢特征的研究，对于胎儿体外循环相关研究具有重要的学术与应用价值。