Transcriptional regulation of the germline piRNA pathway in the Drosophila ovary [ChIP-Seq]

The gene-regulatory mechanisms behind the female germline piRNA pathway in ovaries of metazoan species remain largely unexplored. Here, using the Drosophila model and combinations of ChIP-seq, RNA-seq, ATAC-seq, and single-cell approaches, we reveal the transcription factors that regulate the female germline piRNA pathway in animal ovaries. Ectopic Ovo ChIP-seq after enforced ovo expression in ovarian somatic cells (OSCs). Characterization of the ectopic Ovo binding sites following enforced ovo expression in OSCs后生动物（metazoan）卵巢内雌性生殖系piRNA通路（piRNA pathway）的基因调控机制，目前仍未得到充分探索。本研究以果蝇（Drosophila）为模型，结合染色质免疫沉淀测序（ChIP-seq）、RNA测序（RNA-seq）、转座酶可及性测序（ATAC-seq）及单细胞技术，揭示了调控动物卵巢内雌性生殖系piRNA通路的转录因子。在卵巢体细胞（ovarian somatic cells, OSCs）中强制过表达ovo基因后，开展异位Ovo的染色质免疫沉淀测序（ChIP-seq）实验。对在卵巢体细胞中强制过表达ovo基因后的异位Ovo结合位点进行特征分析。