Differential miRNAs between Sensitive and Resistant Pig to Escherichia coli F18 by deep sequencing. Sus scrofa

An M307 G/A point mutation of FUT1 gene has been considered as a usful marker to select the piglets that are sensitive(GG/AG genotype) or resistant(AA) to Escherichia coli F18 in foreign pig breeds. However,it is not suitable to Chinese native breeds. Duodenal tissues were collected from 3 full-sib pairs of Sutai pigs (a new hybrid between the Duroc and Taihu breeds) at the age of 28-day differing in adhesion phenotype to find the differential miRNAs that can provide the basis for analyzing the different mechanisms of E.coli F18 resistance between foreign and Chinese native breeds,as well as for breeding for disease resistance in Chinese native breeds in the future. Overall design: piglets at the age of 28-day sensitive(GG genotype) to Escherichia coli F18 vs resistant(AA) ones.Biological replicates:3 full-sib pairs of Sutai pigs(3 pairs for GG/AA genotype of FUT1 gene).One GG sample (512 piglet) was sequenced twice to verify the result. 2385 piglet and 2383 piglet were a pair of full-sib with AA and GG genotype at M307 positionof FUT1 gene, respectively. Unfortunately, the sequencing of 2385 was a failure and was excluded. 2385 piglet has an AA genotype of the FUT1 gene

FUT1基因的M307 G/A点突变曾被视为筛选外来猪品种中对大肠杆菌F18易感（GG/AG基因型）或耐受（AA基因型）仔猪的有效分子标记，但该标记并不适用于中国地方猪种。本研究采集了28日龄苏太猪（杜洛克与太湖猪杂交培育的新品种）的3个全同胞家系的十二指肠组织，这些家系的仔猪在大肠杆菌F18黏附表型上存在差异，旨在筛选差异表达microRNA（miRNA），为解析外来猪种与中国地方猪种间大肠杆菌F18抗性差异的分子机制提供依据，同时为未来中国地方猪种的抗病育种奠定理论基础。实验整体设计：28日龄大肠杆菌F18易感（GG基因型）与耐受（AA基因型）仔猪的对照分析。生物学重复：3个全同胞家系的苏太猪（对应FUT1基因GG/AA基因型的3个家系）。其中1份GG基因型样本（仔猪512）进行了两次测序以验证实验结果。仔猪2385与2383为一对全同胞，二者在FUT1基因M307位点分别为AA基因型与GG基因型。遗憾的是，仔猪2385的测序实验失败，其数据被排除；而仔猪2385实为FUT1基因AA基因型。