Identification of significantly differential expression of long noncoding RNAs and pathways in hepatic fibrosis rat liver issues. Identification of significantly differential expression of long noncoding RNAs and pathways in hepatic fibrosis rat liver issues

Objective: To identify the long noncoding (lncRNA) and its potential roles in hepatic fibrosis rat liver issues induced by CCl4. Methods: LncRNAs and genes were analyzed in fibrosis rat liver issues by RNA sequencing and verified by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Differential expressed lncRNAs and genes were subjected to bioinformatics analysis and involved to construct co-expression network. Overall design: LncRNAs and genes were analyzed in 3 fibrosis rat liver issues and 3 control rat liver issues by RNA sequencing.

研究目标：鉴定四氯化碳（CCl₄）诱导的肝纤维化大鼠肝组织中的长链非编码RNA（long noncoding RNA, lncRNA）及其潜在作用。研究方法：通过RNA测序对纤维化大鼠肝组织中的长链非编码RNA与基因进行分析，并采用定量逆转录聚合酶链反应（quantitative reverse transcription polymerase chain reaction, qRT-PCR）进行验证；对差异表达的长链非编码RNA与基因开展生物信息学分析，并用于构建共表达网络。实验设计：通过RNA测序对3份纤维化大鼠肝组织与3份对照大鼠肝组织中的长链非编码RNA与基因进行分析。