Raw sequence reads from multiplex PCR amplicon sequencing of Ixodes scapularis nymphs

This dataset consists of 175 host seeking Ixodes scapularis nymphs sequenced with a multiplex PCR amplicon sequencing assay (MPAS). All tick specimens were morphologically identified. This dataset was used to validate a custom bioinformatics pipeline used to analyze MPAS sequences. The assay contained primers targeting the four genera associated with Ixodes transmitted human pathogens: Borrelia spp., Babesia spp., Anaplasma spp., and Ehrlichia spp.本数据集包含175只宿主搜寻型肩突硬蜱（Ixodes scapularis）若虫，采用多重PCR扩增子测序检测（multiplex PCR amplicon sequencing assay, MPAS）完成测序。所有蜱虫标本均经形态学鉴定。本数据集用于验证一款用于分析MPAS序列的定制化生物信息学分析流程。该检测所用引物靶向与硬蜱传播人类病原体相关的四个菌属：疏螺旋体属（Borrelia spp.）、巴贝斯虫属（Babesia spp.）、无形体属（Anaplasma spp.）以及埃里希体属（Ehrlichia spp.）