Multidimensional Impact of Microbiota Absence on Thymic T Cell Development in Mice: A Study Based on Single-Cell and Spatial Transcriptomics

This study utilized single-cell transcriptomics and spatial transcriptomics techniques to systematically compare thymic cellular composition and gene expression characteristics between germ-free mice and specific pathogen-free mice at different developmental stages (0, 2, 4, and 10 weeks of age).