RT-PCR and PCR primers.

RT-PCR primers and PCR primers used for r10001, r04103, cy0428, and cy0575 are listed. Replicates for cy0428 and cy0575 were separated following vRNA isolation from plasma, while replicates for r10001 and r04103 were separated following RT-PCR. Replicates were included wherever they were completed. PCR primers contained Common Sequence (CS) tags. Primer sequences, along with two different common sequence tags, were incorporated in different variations.

本研究列出了用于r10001、r04103、cy0428及cy0575的逆转录聚合酶链反应（Reverse Transcription Polymerase Chain Reaction，RT-PCR）引物与聚合酶链反应（Polymerase Chain Reaction，PCR）引物。cy0428与cy0575的生物学重复样本在从血浆中分离病毒RNA（viral RNA, vRNA）后完成分组，而r10001及r04103的生物学重复样本则在完成逆转录聚合酶链反应后进行分组。所有已完成的生物学重复样本均被纳入本次研究。聚合酶链反应引物带有通用序列（Common Sequence, CS）标签，引物序列与两种不同的通用序列标签以不同变体形式完成整合。