Crystal structure and anti-breast cancer activity evaluation of a nanosized bismuth(V)-containing coordination complex based on the F-decorated ligand

In the current study, a new heteroleptic triorganobismuth (V) complex with the chemical formula of Bi(phenyl)3(2,3-dfa)2 has been successfully prepared by using C donor phenyl ring and O donor 2,3-difluorobenzoic acid (2,3-H2dfa) ligands, which has been fully characterized by single crystal X‐ray crystallography, elemental analysis, powder X-ray diffraction along with the thermogravimetric analysis. Furthermore, a green hand grinding technique was used to get the nanocrystals of 1 with an average size of 200 nm as revealed via the scanning electron microscopy (SEM). The inhibitory effect of BT474 cells proliferation after nano 1 treatment was detected with CCK-8 assay. The apoptosis of BT474 cells was measured with FITC-Annexin V/PI double staining assay, the influence on ROS accumulation was determined with DCFH-DA staining.本研究中，以碳供体苯基环与氧供体2,3-二氟苯甲酸(2,3-H2dfa)为配体，成功合成了一种新型杂配型三有机铋(V)配合物，其化学式为Bi(苯基)3(2,3-dfa)2。该配合物通过单晶X射线衍射、元素分析、粉末X射线衍射以及热重分析进行了全面表征。此外，采用绿色手工研磨法制备得到该配合物的纳米晶体，经扫描电子显微镜(SEM)表征可知其平均粒径为200 nm。通过CCK-8法检测了纳米级样品1处理后对BT474细胞增殖的抑制作用；采用FITC-Annexin V/PI双染色法检测了BT474细胞的凋亡情况；利用DCFH-DA染色法测定了其对活性氧(ROS)积累的影响。