Complexome profiling of mitochondrial complexes isolated from seedlings or mature leaves

Assembly of the plant OXPHOS complexes was studied using complexome profiling. mitochondria were extracted from seedlings or mature leaves of the reference organism Arabidopsis thaliana. In seedlings, a fast-growing tissue, mitochondria biogenesis is very active whereas in mature leaves, little biogenesis is occurring. Comparison of both datasets allow the identification of assembly intermediates that are accumulating in seedlings but not in leaves.本研究借助复合物组分析（complexome profiling）技术，探究植物氧化磷酸化（OXPHOS）复合物的组装过程。实验从模式生物拟南芥（Arabidopsis thaliana）的幼苗与成熟叶片中提取线粒体。幼苗作为快速生长的组织，其线粒体生物发生过程极为活跃；而成熟叶片中的线粒体生物发生活动极为微弱。通过对比两组数据集，可鉴定出仅在幼苗中富集、却未在成熟叶片中出现的组装中间体。