Gene expression profile of B55a/PPP2R2A knocked-down Parkin-expressing HeLa cells

Or study identified the PP2A-B55a/PPP2R2A Ser-Thr phosphatase as a regulator of Parkin-dependent mitophagy and mitochondrial biogenesis. HeLa cells expressing Parkin and knocked-down for B55a/PPP2R2A induce a lysosome- and mitochondria-related transcriptional programmes down-stream TFEB and PGC-1a transcription factor activation. Total RNA was extracted from B55a/PPP2R2A kncked-down cells, induced for Parkin expression with Doxycycline. Cells transiently transfected with a control oligo were used a control sample.

本研究鉴定出蛋白磷酸酶2A-B55α/PPP2R2A（PP2A-B55a/PPP2R2A）丝氨酸/苏氨酸磷酸酶，其可作为帕金蛋白(Parkin)依赖的线粒体自噬与线粒体生物发生的调控因子。表达帕金蛋白且敲低B55α/PPP2R2A的海拉细胞，可激活TFEB与PGC-1α转录因子，进而诱导溶酶体及线粒体相关的下游转录程序。研究人员从经多西环素(Doxycycline)诱导帕金蛋白表达的B55α/PPP2R2A敲低细胞中提取总RNA，并以转染对照寡核苷酸序列的瞬时转染细胞作为对照样本。