Gene expression of ulcer-associated cell lineage

We applied RNA-seq analysis of total RNA isolated from laser capture microdissected intestinal epithelium. The analysis aimed at charactericing the gene expression seen in ulcer-associated cell lineage epithelial cells, and to contrast this with that of healthy control epithelium and epithelium from inflammatory bowel disease-patients with active inflammation. The epithelial monolayer from six patients with active Crohn's disease (CD) with ulcer-associated cell lineage (UACL) and seven healthy controls were included in the final analysis. Both UACL and non-UACL epithelium was isolated from the CD patients, bringing the total sample pool up to 19. ***Please note that raw data is not provided as Norwegian law does not allow public access to human sequences raw data.本研究对经激光捕获显微切割（Laser Capture Microdissection, LCM）分离得到的肠道上皮细胞总RNA进行了RNA测序（RNA-seq）分析。本分析旨在表征溃疡相关细胞谱系（Ulcer-Associated Cell Lineage, UACL）上皮细胞的基因表达特征，并将其与健康对照上皮组织，以及伴活动性炎症的炎症性肠病（Inflammatory Bowel Disease, IBD）患者上皮组织的基因表达谱进行对比。最终分析纳入了6例伴UACL的活动性克罗恩病（Crohn's Disease, CD）患者的上皮单层细胞，以及7例健康对照个体的上皮组织。研究人员从每位克罗恩病患者体内分别分离获取UACL上皮与非UACL上皮样本，最终总样本量达19例。***请注意：由于挪威法律不允许人类测序原始数据的公开获取，本数据集未提供原始测序数据。