DataSheet2_Serum Metabolomics Coupling With Clinical Laboratory Indicators Reveal Taxonomic Features of Leukemia.PDF

Metabolic abnormality has been considered to be the seventh characteristic in cancer cells. The potential prospect of using serum biomarkers metabolites to differentiate ALL from AML remains unclear. The purpose of our study is to probe whether the differences in metabolomics are related to clinical laboratory-related indicators. We used LC-MS-based metabolomics analysis to study 50 peripheral blood samples of leukemia patients from a single center. Then Chi-square test and T test were used to analyze the clinical characteristics, laboratory indicators and cytokines of 50 patients with leukemia. Correlation analysis was used to explore the relationship between them and the differential metabolites of different types of leukemia. Our study shows that it is feasible to better identify serum metabolic differences in different types and states of leukemia by metabolomic analysis on existing clinical diagnostic techniques. The metabolism of choline and betaine may also be significantly related to the patient’s blood lipid profile. The main enrichment pathways for distinguishing differential metabolites in different types of leukemia are amino acid metabolism and fatty acid metabolism. All these findings suggested that differential metabolites and lipid profiles might identify different types of leukemia based on existing clinical diagnostic techniques, and their rich metabolic pathways help us to better understand the physiological characteristics of leukemia.代谢异常已被视为癌细胞的第七大特征。利用血清生物标志物代谢物区分急性淋巴细胞白血病（ALL, Acute Lymphoblastic Leukemia）与急性髓系白血病（AML, Acute Myeloid Leukemia）的潜在应用前景仍不明确。本研究旨在探讨代谢组学差异是否与临床实验室相关指标存在关联。本研究采用基于液相色谱-质谱联用（LC-MS, Liquid Chromatography-Mass Spectrometry）的代谢组学分析方法，对来自单中心的50份白血病患者外周血样本进行研究。随后通过卡方检验与t检验，分析50例白血病患者的临床特征、实验室指标及细胞因子，并借助相关性分析探究上述指标与不同类型白血病差异代谢物之间的关联。本研究结果显示，依托现有临床诊断技术开展代谢组学分析，可更好地识别不同类型及状态白血病患者的血清代谢差异。胆碱与甜菜碱的代谢过程或与患者的血脂谱显著相关。区分不同类型白血病差异代谢物的主要富集通路为氨基酸代谢与脂肪酸代谢。上述所有研究结果表明，依托现有临床诊断技术，可通过差异代谢物与血脂谱区分不同类型的白血病，而其丰富的代谢通路有助于我们更深入地理解白血病的生理学特征。