mRNA seq of UEA-1+ Ass+ and UEA-1- Ass+ antigen sampling cells. Oncorhynchus mykiss

We aimed to characterize antigen sampling cells which uptake Aeromonas salmonicida subsp salmonicida (Ass) bacterin in the gill epithelium of rainbow trout. There are two phenotypes of the antigen sampling cells in the gill epithelium: UEA-1+Ass+ and UEA-1-Ass+ cells. We sorted the three cell fractions (UEA-1+Ass+, UEA-1-Ass+ and totally negative) from gill-epithelial cells of fish bath-vaccinated with Ass bacterin.

本研究旨在鉴定虹鳟（rainbow trout）鳃上皮中摄取杀鲑气单胞菌杀鲑亚种（Aeromonas salmonicida subsp. salmonicida, Ass）菌苗的抗原采样细胞。该鳃上皮内的抗原采样细胞存在两种表型：UEA-1⁺Ass⁺与UEA-1⁻Ass⁺细胞。我们从经Ass菌苗水浴免疫的虹鳟鳃上皮细胞中分选获得三类细胞组分：UEA-1⁺Ass⁺、UEA-1⁻Ass⁺及完全阴性细胞。