Interferon-gamma is Quintessential for NOS2 and COX2 Expression in ER- Breast Tumors that Lead to Poor Outcome. Interferon-gamma is Quintessential for NOS2 and COX2 Expression in ER- Breast Tumors that Lead to Poor Outcome

Previously, we have proved the NOS2 and COX2 genes play an important role in ER-negative breast cancer. In this study, we utilized the scRNAseq technique to investigate how cancer cells react to cytokines with and without IFNG combination treatments. Overall design: Synchronized MDA-MB-231 cells were treated with cytokines with and without IFNG for 48 hours

既往研究中，我们已证实一氧化氮合酶2（NOS2）与环氧合酶2（COX2）在雌激素受体阴性乳腺癌（ER-negative breast cancer）中发挥关键作用。本研究采用单细胞RNA测序（scRNAseq）技术，旨在探究癌细胞在联合干扰素γ（IFNG）与未联合IFNG的细胞因子处理下的应答情况。整体实验设计：将同步化的MDA-MB-231细胞分别使用含IFNG与不含IFNG的细胞因子处理48小时。