Use of aqueous extract of noni in extender for sheep semen freezing

ABSTRACT The study aimed to evaluate the action of aqueous extract of noni in an extender for sheep semen freezing. Treatments differed in inclusion of aqueous extract of noni in the extender: T1 ˗ no addition; T2 ˗ 24µg/mL; T3 ˗ 72µg/mL; and T4 ˗ 120µg/mL. Ejaculates were collected, diluted in the four treatments, and frozen. After thawing, the semen was subjected to a thermoresistance test and evaluated for subjective motility, vigor, membrane integrity assessment by hypo-osmotic swelling test, live-dead assay, computer-assisted sperm analysis and the status of sperm capacitation and acrosome reaction. Data were subjected to ANOVA, and then to Student Newman Keuls’s test at 5% significance level. In the thermoresistance test after two hours of incubation, motility in T4 (120µg/mL) was lower than in the other treatments, with no differences in the HoS test in either diluted semen or in the semen evaluated immediately post-thawing, while for the other times, treatments showed similar responses. Regarding the motility parameters, a difference was observed for progressive motility, curvilinear velocity, average path velocity, and amplitude of lateral head displacement. As to the sperm capacitation status, a difference was observed between treatments for the sperm capacitated with intact acrosome.

摘要 本研究旨在评估诺丽（noni）水提物在绵羊精液冷冻稀释液中的应用效果。试验设置4组不同诺丽水提物添加浓度的稀释液处理组：T1组不添加；T2组添加24μg/mL；T3组添加72μg/mL；T4组添加120μg/mL。采集精液样本，分别经4组稀释液处理后进行冷冻保存。解冻后，对精液开展耐热性试验，并对以下指标进行评估：精子主观运动评分、精子活力、低渗肿胀试验（hypo-osmotic swelling test）检测膜完整性、死活染色检测法、计算机辅助精子分析，以及精子获能与顶体反应状态。数据采用单因素方差分析（analysis of variance, ANOVA）进行统计检验，随后以5%显著性水平实施Student-Newman-Keuls多重比较检验。孵育2小时后的耐热性试验结果显示，T4组（120μg/mL）的精子运动率低于其余各组；稀释后精液及解冻后即刻检测的样本中，低渗肿胀试验结果无组间显著差异；其余检测时间点各组响应趋势相近。在精子运动参数方面，前向运动率、曲线运动速度、平均路径速度及头部侧摆幅度均存在组间显著差异。针对精子获能状态，顶体完整的获能精子比例在各组间存在显著差异。