RNA-sequencing to idendify axon RNAs in dentate gyrus granule cells, dorsal root ganglion cells and retinal ganglion cells. RNA-sequencing to idendify axon RNAs in dentate gyrus granule cells, dorsal root ganglion cells and retinal ganglion cells

To analyze axon transcriptome of dentate gyrus granule cells, dorsal root ganglion cells and retinal ganglion cells, we perfomed axon RNA sequencing.Neurons were cultured in microfluidic chambers and axons were isolated. Total RNA of axons was collected separately and subjected to library construction using M01440v2 NuGEN Trio RNA-seq kit. After sequencencing,we used TPM method to normalize RNA-seq reads. Overall design: Three axon samples from different cells as experimental group.

为分析齿状回颗粒细胞、背根神经节细胞与视网膜神经节细胞的轴突转录组，我们开展了轴突RNA测序实验。我们将神经元培养于微流控室（microfluidic chambers）中并分离轴突，随后分别收集轴突总RNA，使用M01440v2 NuGEN Trio RNA-seq试剂盒完成文库构建。测序完成后，采用TPM（Transcripts Per Million）法对RNA测序读段（reads）进行归一化处理。实验设计概况：设置3组来源于不同细胞的轴突样本作为实验组。