Evaluation of Boerhavia diffusa for neuroprotection, cytotoxicity and monoamine oxidase inhibition in SH-SY5Y cells and a Zebrafish model

The dataset supports research of a proprietary methanolic Boerhavia diffusa crude extract, examined for its inherent cytotoxicity and neuroprotection against 6-hydroxydopamine (6-OHDA)-induced damage in neuroblastoma SH-SY5Y cell line, assessed using the sulforhodamine B (SRB) assay. Antioxidant activity was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. Monoamine oxidase (MAO) inhibition was assessed using the Amplex Red™ monoamine oxidase assay kit. Assessment of zebrafish viability and developmental outcomes following exposure to BD and co-treatment with 6-OHDA was determined using zebrafish larvae of Data was analysed using Graphpad Prism 8.02 (San Diego, CA). Outliers were identified and excluded using the ROUT (Q=1%) method. At least three technical and experimental replicates were used (n ≥ 9). Groups were compared using analysis of variance (ANOVA), and the Kruskal Wallis ANOVA with Dunn’s multiple comparisons test for non-parametric data. The indicator of significance was p post hoc testing for parametric data, or the Kruskal-Wallis ANOVA with Dunn’s multiple comparisons test for non-parametric data. Biological replicates of a minimum of n = 12 was employed for each treatment condition.本数据集可用于支撑针对专利性甲醇提取物白花黄细心（Boerhavia diffusa，下文简称BD）粗提物的相关研究。该粗提物的固有细胞毒性，以及其对6-羟基多巴胺(6-OHDA)诱导的神经母细胞瘤SH-SY5Y细胞系损伤的神经保护作用，通过磺酰罗丹明B(SRB)实验完成评估。其抗氧化活性通过2,2-二苯基-1-苦基肼(DPPH)实验与铁离子还原抗氧化能力(FRAP)实验进行测定。单胺氧化酶(MAO)抑制活性则采用Amplex Red™单胺氧化酶检测试剂盒完成评估。针对暴露于BD并联合6-OHDA处理的斑马鱼幼鱼存活率与发育结局的评估，以斑马鱼幼鱼为实验材料开展。所有实验数据均采用GraphPad Prism 8.02（美国加利福尼亚州圣地亚哥）进行分析。采用ROUT(Q=1%)法识别并剔除异常值。细胞实验至少设置3次技术重复与生物学重复（n≥9）；斑马鱼实验各处理组均设置至少12次生物学重复（n≥12）。组间比较时，参数化数据采用方差分析(ANOVA)，非参数化数据采用克鲁斯卡尔-沃利斯方差分析结合邓恩多重比较检验；参数化数据的显著性以事后检验后的p值为判定标准，非参数化数据则采用该多重比较检验方法。