ORC1 binds to cis-transcribed RNAs for efficient activation of human origins [CDC45_ChIP-seq]

By investigating the RNA binding activity of human ORC1, we show that it interacts with RNAs transcribed at active origins, which is linked to ORC1 dynamic association to the chromatin and optimal origin firing in S phase. Overall design: CDC45 ChIP DNA purification and sequencing from untreated HCT116 cells (stably expressing WT or MUT-ORC1) in duplicate本研究通过探究人类ORC1的RNA结合活性，证实其可与活跃复制原点处转录的RNA相结合，该过程与ORC1在染色质上的动态结合以及S期内复制原点的高效激活密切相关。 总体实验设计：对未处理的、稳定表达野生型（Wild Type, WT）或突变型ORC1（Mutant ORC1, MUT-ORC1）的HCT116细胞，开展细胞分裂周期蛋白45（CDC45）染色质免疫共沉淀（Chromatin Immunoprecipitation, ChIP）的DNA纯化与测序实验，实验设置两份生物学重复。