Quantitative Proteomic Analysis of Residual Host Cell Protein Retention Across Adeno- Associated Virus (AAV) Affinity Chromatography

To better understand host cell protein (HCP) retention in adeno-associated virus (AAV) downstream processes, sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH-MS) was used to quantitatively profile residual HCPs for four AAV serotypes (2, 5, 8, and 9) produced with HEK293 cells and purified using POROS™ CaptureSelect™ AAVX affinity chromatography.

为深入解析腺相关病毒（adeno-associated virus，AAV）下游工艺中的宿主细胞蛋白（host cell protein，HCP）残留规律，本研究采用全理论片段离子质谱连续窗口采集法（sequential windowed acquisition of all theoretical fragment ion mass spectra，SWATH-MS），对以HEK293细胞制备、并经POROS™ CaptureSelect™ AAVX亲和层析纯化的4种血清型（2、5、8及9型）腺相关病毒的残留宿主细胞蛋白进行定量表征。