Coordinates of TCP-seq reads, mapped to yeast mRNAs.

Dynamics of ribosome scanning and recycling revealed by translation complex profiling. Dataset contains alignment information of RNA-seq reads produced in TCP-seq in yeast, mapped to a reference consisting of spliced transcript sequences (SacCer2) plus up to 1000nt flanking sequence.<br><br>Description of column headers:<br>gene: The gene to whose transcript the read was mapped. read: Unique read identifier (some reads mapped more than once)<br>start: The start position of the read relative to the gene's annotated start codon ('A' in AUG set to zero; all coordinates are inclusive of the end-nucleotides in the FPs) <br>min_end: The 5'-most possible position of the RNA fragment's 3' end. In cases of an ambiguity caused by the end falling on an oligo-A tract, this will be less than max_end. <br>max_end: The 3'-most possible position of the RNA fragment's 3' end. In cases of an ambiguity caused by the end falling on an oligo-A tract, this will be greater than min_end. sample: The sample / fraction from which the library was generated.<br>

通过翻译复合体谱分析揭示核糖体扫描与循环动力学。本数据集包含酵母中通过TCP-seq实验获取的RNA测序（RNA-seq）读段的比对信息，这些读段被比对至由剪接转录本序列（SacCer2）加上至多1000nt侧翼序列组成的参考序列。

列表头说明如下：
gene（基因）：该读段比对至其转录本的目标基因
read（读段）：唯一读段标识符（部分读段存在多次比对情况）
start（起始位置）：相对于基因注释起始密码子的读段起始位置（AUG密码子中的'A'被设为0；所有坐标均包含FPs中的末端核苷酸）
min_end（最小末端位置）：RNA片段3'端的最靠左可能位置。若末端落在寡聚A（oligo-A）区域导致比对歧义，则该值小于max_end
max_end（最大末端位置）：RNA片段3'端的最靠右可能位置。若末端落在寡聚A区域导致比对歧义，则该值大于min_end
sample（样本）：生成该文库的样本/组分