Glycogen synthase kinase-3 beta inhibitors protectagainst the acute lung injuries resulting from acute necrotizing pancreatitis

Abstract Purpose The research is intended for clarification of the efficacy as well as the underlying mechanism of GSK-3β inhibitors on the advancement of acute lung injuries in acute necrotizing pancreatitis (ANP) in rats. Methods Seventy-two rats were randomly divided into 6 groups: (1)ANP-vehicle; (2)ANP-TDZD-8;(3)ANP-SB216763;(4)Sham-vehicle;(5)Sham-TDZD-8;(6)Sham-SB216763; Blood biochemical test, histopathological examination and immunohistochemical analysis of rats pancreas and lung tissues were performed. The protein expression of GSK-3β, phospho-GSK-3β (Ser9), iNOS, ICAM-1, TNF-α, and IL-10 were detected in lung tissues by Western-blot. Results The outcomes revealed that the intervention of GSK-3β inhibitors alleviated the pathological damage of pancreas and lung (P摘要 目的 本研究旨在明确糖原合成酶激酶3β（GSK-3β）抑制剂对大鼠急性坏死性胰腺炎（ANP）继发急性肺损伤进展的疗效及其潜在作用机制。方法 将72只大鼠随机分为6组：(1)急性坏死性胰腺炎-溶剂对照组（ANP-vehicle）；(2)急性坏死性胰腺炎-TDZD-8组；(3)急性坏死性胰腺炎-SB216763组；(4)假手术-溶剂对照组（Sham-vehicle）；(5)假手术-TDZD-8组；(6)假手术-SB216763组。对大鼠胰腺及肺组织开展血液生化检测、病理组织学检查与免疫组化分析。采用蛋白质印迹法（Western-blot）检测肺组织中GSK-3β、磷酸化GSK-3β（Ser9）、诱导型一氧化氮合酶（iNOS）、细胞间黏附分子1（ICAM-1）、肿瘤坏死因子α（TNF-α）以及白细胞介素10（IL-10）的蛋白表达水平。结果 研究结果显示，GSK-3β抑制剂干预可减轻胰腺与肺组织的病理性损伤（P