Run or die in the evolution of de novo microRNAs. Run or die in the evolution of de novo microRNAs

MicroRNAs (miRNAs) are a class of small, endogenous RNAs that regulate mRNA expressions post-transcriptionally. To study their expression, we collected and sequenced small RNA libraries in Drosophila species. To survey their regulation divergence, we dissected testes from miR-983 mutant and control flies in two sibling species and performed an RNA-seq analysis. Overall design: Two small RNA samples from D. erecta and D. sechellia testes as well as eight RNA-seq samples from D. melanogaster and D. simulans testes were analyzed. For RNA-seq, each genotype contains two biological replicates.

微小RNA（MicroRNAs，miRNAs）是一类内源性小型RNA，可在转录后水平调控信使RNA（mRNA）的表达。为研究其表达模式，我们收集了果蝇属（Drosophila）物种的小型RNA文库并进行测序。为探究其调控差异，我们选取两个近缘果蝇物种，分别解剖其miR-983突变体与对照果蝇的睾丸，并开展RNA测序（RNA-seq）分析。整体实验设计：本研究共分析两类样本，分别为来自直立果蝇（D. erecta）与塞舌尔果蝇（D. sechellia）睾丸的2个小型RNA样本，以及来自黑腹果蝇（D. melanogaster）与拟暗果蝇（D. simulans）睾丸的8个RNA-seq样本。针对RNA-seq样本，每种基因型均设置2个生物学重复。