Gene expression in bone marrow-derived dendritic cells obtained from wild-type mice or 3BP2 DL/DL mice stimulated with or without curdlan

C-type lectin dectin-1 is known to recognize beta-glucans such as curdlan and propagate intracellular signaling for activation of NF-kappa B to induce anti-fungal immunity. Recently, we found that adaptor protein 3BP2 is required for the dectin-1-induced activation of NF-kappa B. In this study, we performed microarray analysis to identify genes of which expression is regulated by dectin-1 and 3BP2. Bone marrow-derived dendritic cells obtained from wild-type mice (WT cells) or 3BP2 DL/DL mice (DL cells) were stimulated with (Cd+) or without (Cd-) curdlan for 4 h. After cells were washed with PBS, total RNA was extracted and subjected to microarray analysis.

已知C型凝集素dectin-1（C-type lectin dectin-1）可识别包括柯德兰（curdlan）在内的β-葡聚糖（beta-glucans），通过传递胞内信号激活核因子κB（NF-kappa B），进而诱导抗真菌免疫。近期本研究证实，接头蛋白3BP2（adaptor protein 3BP2）是dectin-1介导的核因子κB激活所必需的蛋白。本研究通过微阵列分析（microarray analysis），筛选受dectin-1与3BP2共同调控的基因。实验中，将从野生型小鼠（WT细胞）或3BP2 DL/DL小鼠（DL细胞）分离的骨髓源树突状细胞，以含（Cd+）或不含（Cd-）柯德兰的体系刺激4小时。细胞经磷酸盐缓冲液（PBS）洗涤后，提取总RNA并开展微阵列分析。