RNA-seq analysis of SMYD2-silenced prostate cancer cells C4-2 and ChIP-seq analysis for both SMYD2-silenced and SMYD2-overexpressed C4-2 cells

Total RNA was obtained from C4-2 cells with or without SMYD2 silence to perform a high-throughput sequencing to explore the expression profile changes caused by SMYD2 in prostate cancer. AR chromatin immunoprecipitation was conducted using SMYD2-silenced, SMYD2-overexpressed, and parent C4-2 cells. Then, the chromatin immunoprecipitates were extracted to perform high-throughput sequencing to investigate the influence of SMYD2 on AR genomic binding profiles.

本研究从经SMYD2基因沉默或未经SMYD2基因沉默的C4-2细胞中提取总RNA，通过高通量测序（high-throughput sequencing）探究SMYD2对前列腺癌基因表达谱的调控变化。此外，分别以SMYD2基因沉默、SMYD2过表达及亲本C4-2细胞为实验材料，开展针对AR（androgen receptor，雄激素受体）的染色质免疫共沉淀（chromatin immunoprecipitation）实验；随后收集染色质免疫共沉淀复合物并进行高通量测序，以解析SMYD2对AR基因组结合谱的调控作用。