RNAseq data for hybrid Formica genome annotation - FASTQ 1 (Forward)

This RNAseq data was generated from nine wood ant individuals originating from six nests in the Långholmen population, which is of hybrid <i>Formica aquilonia</i> x <i>Formica polyctena</i> ancestry (R lineage: nest FA4, 3 individuals and W lineage: nest FA15, 1 ind.; FA17, 2 inds.; FA25, 1 ind.; FA35, 1 ind.; FAu2014a, 1 ind.). These individuals were at different larval stages and total RNA was extracted from whole bodies using an ALLPrep DNA/RNA Mini Kit (Qiagen) following manufacturer’s instructions. Individual RNA qualities were assessed using a Bioanalyzer (Agilent 2100). Libraries were constructed using NEBNext Ultra RNA Library Prep Kits and samples were sequenced on an Illumina NextSeq platform (paired-end mode, 150 bp) at the Biomedicum Functional Genomics Unit (FuGU, University of Helsinki). Raw reads were trimmed using Trimmomatic (v0.35, parameters LEADING:20 TRAILING:20 SLIDINGWINDOW:5:20 MINLEN:50 Bolger, Lohse, and Usadel 2014) and unpaired reads were discarded.Approximately 5.60 million 150bp paired reads were randomly sampled per individual and combined into two (Forward and Reverse) FASTQ files, totaling 50 million paired reads over all individuals.

本转录组测序（RNAseq）数据源自朗霍尔曼（Långholmen）种群的6个蚁巢的9只木蚁个体，该种群为红蚁（*Formica aquilonia*）与多栉蚁（*Formica polyctena*）的杂交种群：其中R谱系包含FA4蚁巢的3个个体；W谱系包含FA15蚁巢（1个个体）、FA17蚁巢（2个个体）、FA25蚁巢（1个个体）、FA35蚁巢（1个个体）及FAu2014a蚁巢（1个个体）。这些个体处于不同的幼虫发育阶段，研究人员依照试剂盒制造商的说明书，使用QIAGEN公司的ALLPrep DNA/RNA Mini Kit从全虫体中提取总RNA。采用安捷伦2100生物分析仪（Agilent 2100）对单样本RNA质量进行质检。使用NEBNext Ultra RNA文库制备试剂盒构建测序文库，随后在赫尔辛基大学生物医学功能基因组学单元（Biomedicum Functional Genomics Unit，简称FuGU）的Illumina NextSeq测序平台上以双端模式（150bp读长）完成测序。使用Trimmomatic（v0.35，参数设置为LEADING:20、TRAILING:20、SLIDINGWINDOW:5:20、MINLEN:50，参考Bolger、Lohse与Usadel 2014年的研究）对原始测序读段进行修剪，并丢弃单端测序读段。对每个个体随机抽取约560万条150bp双端测序读段，合并为正向（Forward）与反向（Reverse）两个FASTQ文件，所有个体的总双端测序读段共计5000万条。