Isolation of extracellular vesicles by PROSPR

Here, we report a novel and inexpensive method of rapidly isolating EVs from small volumes of human blood plasma by PRotein Organic Solvent PRecipitation (PROSPR). PROSPR encompasses a rapid three-step protocol to remove soluble proteins from plasma via precipitation in cold acetone, leaving the lipid-encapsulated EVs behind in suspension. This generates higher purity EVs that can then be obtained from filtration or classical ultracentrifugation methods.本研究报道了一种基于蛋白质有机溶剂沉淀法（Protein Organic Solvent Precipitation, PROSPR）的新颖且低成本的方法，可从少量人血浆中快速分离细胞外囊泡（Extracellular Vesicles, EVs）。PROSPR包含一套快速三步操作流程，通过在冷丙酮中进行沉淀以去除血浆中的可溶性蛋白，使脂质包被的细胞外囊泡留存于悬浮液中。该方法可获得纯度更高的细胞外囊泡，后续可通过过滤或经典超速离心法分离得到目标囊泡。