Innate and adaptive lymphocytes sequentially shape the gut microbiota and lipid metabolism. Mus musculus

In support of our manuscript investigating the roles of ILCs and T cells in the maintenance of gut hoemostasis, we have performed RNAseq on terminal illeum of mice lacking either all adaptive immune cells (RAG1 -/-), deficient in T cells (TCRalpha -/-), or deficient in T cells but co-housed with wild-type mice and RAG1 -/- mice. Overall design: Tissues from three mice per group were analysed, and the following comparisions were made: RAG1-/- vs. WT C57BL/6 and TCRa-/- co-housed vs TCRa-/- seperately housed. Differential expression genes were identified at 1% FDR using DESeq2.

为支持我们关于固有淋巴细胞（ILCs）和T细胞在肠道稳态维持中作用的研究论文，我们对三类小鼠的末端回肠组织开展了RNA测序（RNAseq）：分别为完全缺失适应性免疫细胞的RAG1基因敲除小鼠（RAG1-/-）、T细胞缺陷的TCRα基因敲除小鼠（TCRα-/-），以及T细胞缺陷但与野生型小鼠和RAG1-/-小鼠共同饲养的TCRα-/-小鼠。总体实验设计如下：每组选取3只小鼠的组织进行分析，开展两组对比实验：RAG1-/-小鼠与野生型C57BL/6小鼠的组织对比，以及共同饲养的TCRα-/-小鼠与单独饲养的TCRα-/-小鼠的组织对比。采用DESeq2工具，以1%的错误发现率（FDR）作为阈值鉴定差异表达基因。