Dataset of Cell Growth Regulator with EF-Hand Domain 1 (Cgref1) - a hepatokine study

This dataset contains the original sources of the data included in a manuscript of our group that will be/has been published. The labels of each file are corresponding to the figures described on the manuscript or journal. Individuals may refer to the journal for specific experimental details.

Fig 1C. Liver lysates of CREB-H KO mice injected with AAV-CREB-H-ΔTC or AAV-eGFP.
Fig 1E. Huh7 cell culture medium and/or lysates after transfection of CREB-H-FL, CREB-H-ΔTC and vector control.
Fig 2B. Immunoprecipitation of Cgref1 protein from mouse sera.
Fig 2D. Cgref1 expression in wild type mouse tissues after 3 months of ND or HFD consumption
Fig 2F. Cell culture media and lysates of Hepa1-6 and Huh7 transfected with Cgref1-V5 or CGREF1-V5. IP was performed on cell culture media to capture V5-tagged proteins.
Fig 4C. Akt S473 phosphorylation in eWAT, sWAT and livers of WT and Cgref1-/- mice.
Fig 4D. Akt S473 phosphorylation in eWAT of Cgref1-/- mice intraperitoneally injected with recombinant Cgref1 protein or PBS.
Fig 4F. Analysis of insulin signaling pathway components in eWAT of WT and Cgref1-/- mice.
Fig 4G. Glut4 expression in eWAT of WT and Cgref1-/- mice.
Fig 4H. Glut4 expression in 3T3-L1 preadipocytes after incubation with Cgref1 protein.
Fig 5G. Expression of Acc1 and Scd1 proteins in the liver samples of WT and Cgref1-/- mice.

本数据集涵盖本课题组待发表或已发表论文手稿中所收录数据的原始来源。各文件的标签与该手稿或期刊正文中对应的图表完全匹配。研究者可通过查阅对应期刊获取具体实验细节。
图1C：注射腺相关病毒（Adeno-associated virus, AAV）介导的CREB-H-ΔTC或AAV-eGFP的CREB-H敲除（CREB-H KO）小鼠的肝脏裂解物。
图1E：转染CREB-H-FL、CREB-H-ΔTC及空载对照的Huh7细胞的细胞培养上清及/或细胞裂解物。
图2B：从小鼠血清中免疫沉淀获得的Cgref1蛋白。
图2D：正常饲料（Normal Diet, ND）或高脂饲料（High-Fat Diet, HFD）喂养3个月后，野生型小鼠各组织中的Cgref1表达水平。
图2F：转染Cgref1-V5或CGREF1-V5的Hepa1-6与Huh7细胞的细胞培养上清及细胞裂解物；对细胞培养上清进行免疫沉淀以捕获带有V5标签的蛋白。
图4C：野生型（Wild Type, WT）与Cgref1基因敲除（Cgref1-/-）小鼠的附睾白色脂肪组织（Epididymal White Adipose Tissue, eWAT）、皮下白色脂肪组织（Subcutaneous White Adipose Tissue, sWAT）及肝脏中的Akt S473磷酸化水平。
图4D：腹腔注射重组Cgref1蛋白或磷酸盐缓冲液（Phosphate Buffered Saline, PBS）的Cgref1-/-小鼠附睾白色脂肪组织中的Akt S473磷酸化水平。
图4F：野生型与Cgref1基因敲除小鼠附睾白色脂肪组织中胰岛素信号通路相关组分的分析。
图4G：野生型与Cgref1基因敲除小鼠附睾白色脂肪组织中的Glut4表达水平。
图4H：经Cgref1蛋白孵育后的3T3-L1前脂肪细胞中的Glut4表达水平。
图5G：野生型与Cgref1基因敲除小鼠肝脏样本中Acc1与Scd1蛋白的表达水平。