Expression data from PDE1C knockdown glioblastoma short-term cultures. Homo sapiens

In our lab we detected focal genomic amplification of PDE1C in 90% of short term GBM cultures. Knocking down of PDE1C was associated with compromised capacity opf these cultures to proliferate, migrate and invade. Therefore we carried out affymetric whole genome expression analysis to identify the down stream gene effectors of this function effects. Overall design: IN1472 and IN1760 - 2 GBM short term cultures were transfected with siRNA either for PDE1C (siPD) or non-targeting (siNT) and its down gene effectors were studied.

本实验室在90%的短期胶质母细胞瘤（GBM）原代培养物中检测到PDE1C的局灶性基因组扩增。敲低PDE1C基因会显著削弱这些培养物的增殖、迁移与侵袭能力。因此本研究开展了Affymetrix全基因组表达芯片分析，以鉴定该功能的下游基因效应因子。
整体实验设计：将IN1472与IN1760这2株短期培养的GBM原代细胞分别转染靶向PDE1C的小干扰RNA（siPD）或非靶向对照小干扰RNA（siNT），并对其下游基因效应因子进行研究。