Gene expression patterns associated with Banana Blood Disease (BBD)

Bananas are a globally significant crop, yet varieties like Cavendish and Hom Thong are highly susceptible to banana blood disease (BBD). This study focuses on gene expression patterns in the resistant variety compared to the susceptible following infection with Ralstonia syzygii subsp. celebesensis. The RNA was extracted at 3 time points after innoculation; 12 hr, 1 day, and 7 day. RNA sequencing (RNA-seq) identified serveral genes linked to BBD resistance, showing significant upregulation as early as 12 hours post-inoculation. Enriched molecular processes include hydrolase activity and effector-triggered immunity (ETI) responses, validated by quantitative real-time RT-PCR. These genes offer potential as markers for breeding BBD-resistant banana varieties, supporting efforts toward sustainable banana production.

香蕉是全球重要的农作物，然而卡文迪什（Cavendish）、红香蕉（Hom Thong）等品种对香蕉血液病害（banana blood disease，BBD）具有极高的易感性。本研究聚焦于抗病品种与感病品种在感染青枯拉尔斯顿氏菌西里伯斯亚种（Ralstonia syzygii subsp. celebesensis）后的基因表达模式差异。研究在接种后的3个时间点（12小时、1天和7天）提取总RNA，通过RNA测序（RNA-seq）鉴定出多个与BBD抗性相关的基因，这些基因早在接种后12小时即出现显著上调表达。富集得到的分子生物学过程包括水解酶活性与效应子触发免疫（effector-triggered immunity，ETI）应答，该结果经实时定量反转录聚合酶链式反应（quantitative real-time RT-PCR，qRT-PCR）验证。上述基因可作为培育抗BBD香蕉品种的分子标记，为香蕉可持续生产提供有力支撑。