Maturation status and predicted residuals by rearing treatment and sex.

Sample sizes and percentage of juvenile steelhead sampled at Winthrop National Fish Hatchery in release years 2011–2015 separated according to rearing treatment and sex for maturation status (immature, maturing, mature) and predicted residualism. Categorization of maturity is based on physiological measures (pituitary mRNA expression, follicle stimulating hormone beta-subunit (fshb), luteinizing hormone beta subunit (lhb); testis mRNA expression, anti-Mullerian hormone (amh), insulin-like growth factor-3 (igf3); gonadosomatic index, GSI; and plasma 11-ketotestosterone, 11KT) and their threshold values determined by finite mixture model analysis as described in the Methods section. Categorization of residuals is based on a combination of maturation status and fork length threshold determined by finite mixture model analysis as described in the Methods. Percentages are represented as a portion of total sample size within a release year for a given rearing treatment (S1, S2) and sex (male, female, all fish); these percentages were used to create Figs 8 and 10 (values in bold). (XLSX)2011至2015年放流年度内，于温思罗普国家鱼类孵化场（Winthrop National Fish Hatchery）采集的幼年硬头鳟样本量及占比，按养殖处理方式、性别进行分组，并区分成熟状态（未成熟、发育中、成熟）与预测残留表型。成熟状态的分类依据如下生理检测指标：垂体mRNA表达水平、促卵泡激素β亚基（follicle stimulating hormone beta-subunit，FSHB）、促黄体激素β亚基（luteinizing hormone beta subunit，LHB）；睾丸mRNA表达水平、抗缪勒管激素（anti-Mullerian hormone，AMH）、胰岛素样生长因子-3（insulin-like growth factor-3，IGF3）；性腺指数（gonadosomatic index，GSI）；以及血浆11-酮睾酮（11-ketotestosterone，11KT）。上述指标的阈值由“方法”章节所述的有限混合模型分析确定。残留表型的分类则结合成熟状态与叉长阈值确定，阈值同样由“方法”章节所述的有限混合模型分析得出。本数据集中的占比为对应放流年度内，特定养殖处理组（S1、S2）与性别组（雄性、雌性、全部个体）的样本量占该组总样本量的比例；上述占比数据用于绘制图8与图10（加粗数值即为对应数据）。(XLSX)