Isolation, Absolute Configuration and Cytotoxic Activities of Alkaloids from Hippeastrum goianum (Ravenna) Meerow (Amaryllidaceae)

The phytochemical study of Hippeastrum goianum led to the identification of 13 compounds by means of gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR). Compounds 7-demethoxy-9-O-methylhostasine (1) and 7-deoxi-trans-dihydronarciclasine (2) had their absolute configurations determined by vibrational circular dichroism (VCD). This is the first time that compound 1 is described in the Amaryllidaceae family. The cytotoxicity of all isolated compounds was tested against colorectal carcinoma (HCT 116), breast carcinoma (MCF-7), and non-tumor human retinal pigment epithelium (RPE) cell lines. The half-maximum inhibitory concentration (IC50) of compound 2 against each cell line was equivalent to the positive control (doxorubicin), indicating a considerable cytotoxic activity.本研究对戈亚斯朱顶红（Hippeastrum goianum）开展植物化学分析，通过气相色谱-质谱联用（GC-MS）与核磁共振（NMR）技术，成功鉴定出13种化合物。其中7-去甲氧基-9-O-甲基玉簪素（7-demethoxy-9-O-methylhostasine，编号1）与7-脱氧反式二氢水仙环素（7-deoxi-trans-dihydronarciclasine，编号2）的绝对构型通过振动圆二色谱（VCD）得以明确。这是首次在石蒜科（Amaryllidaceae）植物中报道化合物1。研究团队对所有分离得到的化合物，分别针对结直肠癌细胞（HCT 116）、乳腺癌细胞（MCF-7）及非肿瘤性人视网膜色素上皮（RPE）细胞系开展了细胞毒性测试。结果显示，化合物2对各细胞系的半数抑制浓度（IC50）与阳性对照多柔比星（doxorubicin）相当，表明其具有显著的细胞毒活性。