Dataset for: Real-time self-supervised denoising for high-speed fluorescence neural imaging

General Overview This dataset contains the five data collections used for the analyses in our study, "Real-time self-supervised denoising for high-speed fluorescence neural imaging". It includes newly generated/collected data as well as publicly available datasets that have been reused. The source of each dataset is detailed below. Original Data The following datasets were generated or collected specifically for this study: File 2-Voltage_Simulated_Fast_Dynamics.zip: Simulated data representing fast voltage dynamics. File 5-Calcium_Astrocyte_Volume.tif: Volumetric two-photon imaging data of astrocytes. Publicly Sourced and Reused Data To validate our methods, we also used the following publicly available datasets. We extend our gratitude to the original authors for making their data open. File 1-Calcium_Neuronal_Population.tif: Description: Two-photon calcium imaging of neurons in mouse vS1 at 8 Hz. Original Source: The Neurofinder Challenge. Available at: http://neurofinder.codeneuro.org File 3-Voltage_Single_Cell.zip: Description: Epifluorescence voltage imaging of pyramidal cells (1,000 Hz) with corresponding patch-clamp recordings. Original Publication: Eom, M. et al. Statistically unbiased prediction enables accurate denoising of voltage imaging data. Nat Methods 20, 1581–1592 (2023). DOI: https://doi.org/10.1038/s41592-023-02005-8 File 4-Voltage_Zebrafish.tif: Description: Light-sheet voltage imaging of excitatory neurons (1,000 Hz) in the zebrafish spinal cord. Original Publication: Xie, M.E. et al. High-fidelity estimates of spikes and subthreshold waveforms from 1-photon voltage imaging in vivo. Cell Reports 35, 108954–108954 (2021). DOI: https://doi.org/10.1016/j.celrep.2021.108954 Licensing Information The data in 2-Voltage_Simulated_Fast_Dynamics.zip and 5-Calcium_Astrocyte_Volume.tif are released under the CC-BY 4.0 License. The data in files 1-Calcium_Neuronal_Population.tif, 3-Voltage_Single_Cell.zip, and 4-Voltage_Zebrafish.tif are reused from their original sources and are subject to their respective original licenses. Users should consult the source repositories for specific licensing terms.数据集总览 本数据集包含了我们在研究《用于高速荧光神经成像的实时自监督降噪》中开展分析所使用的五类数据集，涵盖本次研究中新生成/采集的数据以及复用的公开可用数据集。各数据集的来源详情如下文所述。 原始数据 以下数据集均为本研究专属生成或采集： 文件2-Voltage_Simulated_Fast_Dynamics.zip：用于表征快速电压动态的模拟数据。 文件5-Calcium_Astrocyte_Volume.tif：星形胶质细胞的体积双光子成像数据。 公开获取与复用数据集 为验证我们的方法，我们同时使用了以下公开可用数据集。在此感谢原始作者开放其研究数据。 文件1-Calcium_Neuronal_Population.tif： 描述：以8 Hz帧率对小鼠vS1脑区神经元开展的双光子钙成像实验。 原始来源：Neurofinder挑战赛。 获取地址：http://neurofinder.codeneuro.org 文件3-Voltage_Single_Cell.zip： 描述：以1000 Hz帧率采集的锥体细胞落射荧光电压成像数据，附带对应膜片钳记录结果。 原始发表文献：Eom, M. 等人。《统计无偏预测实现电压成像数据的精准降噪》。《自然方法》(Nature Methods)，20卷，1581–1592页(2023年)。 DOI：https://doi.org/10.1038/s41592-023-02005-8 文件4-Voltage_Zebrafish.tif： 描述：以1000 Hz帧率对斑马鱼脊髓内兴奋性神经元开展的光片电压成像。 原始发表文献：Xie, M.E. 等人。《体内单光子电压成像的动作电位与阈下波形高保真估计》。《细胞报告》(Cell Reports)，35卷，108954页(2021年)。 DOI：https://doi.org/10.1016/j.celrep.2021.108954 许可信息 文件2-Voltage_Simulated_Fast_Dynamics.zip与5-Calcium_Astrocyte_Volume.tif中的数据采用知识共享署名4.0(CC-BY 4.0)许可协议发布。文件1-Calcium_Neuronal_Population.tif、3-Voltage_Single_Cell.zip及4-Voltage_Zebrafish.tif中的数据均从原始来源复用，需遵循其各自的原始许可协议。使用者应查阅对应来源仓库以获取具体许可条款。