Table_2_Comprehensive Analysis of Key Genes and Regulatory Elements in Osteosarcoma Affected by Bone Matrix Mineral With Prognostic Values.DOCX

Osteosarcoma is one of the most common types of bone sarcoma with a poor prognosis. However, genes involved in the mineral metabolism in the microenvironment of the bone affected by osteosarcoma are, to date, largely unknown. A public data series (GSE114237) was used to identify differentially expressed genes (DEGs) between osteosarcoma cells adhering to demineralized osseous surfaces and mineralized osseous surfaces. Functional enrichment analysis of DEGs and hub genes, protein-protein interaction network of DEGs and regulatory network (miRNA-mRNA network and transcription factor (TF)-mRNA network), survival analysis of hub genes was visualized. The prognostic hub genes were considered as candidate genes and their functional predictions were analyzed. A total of 207 DEGs were mainly enriched in extracellular space and thirteen hub genes were mainly enriched in the function of epithelial to mesenchymal transition. However, out of these, only one candidate gene was found to be suitable as a candidate gene. Besides that, 297 miRNAs and 349 TFs interacting with the hub genes were screened. In conclusion, the DEGs, hub genes, miRNAs and TFs screened out in this research could contribute to comprehend the latent mechanisms in osteosarcoma affected by matrix mineral and provide potential research molecular for further study.骨肉瘤（Osteosarcoma）是最常见的骨肉瘤类型之一，预后较差。然而，截至目前，骨肉瘤受累骨微环境中参与矿物质代谢的基因大多尚未明确。本研究采用公共数据集（GSE114237），鉴定了黏附于脱矿骨表面与矿化骨表面的骨肉瘤细胞之间的差异表达基因（differentially expressed genes, DEGs）。对DEGs及核心基因（hub genes）开展功能富集分析，构建了DEGs的蛋白质相互作用网络、调控网络（包括microRNA（miRNA）-mRNA调控网络与转录因子（transcription factor, TF）-mRNA调控网络），并对核心基因进行生存分析且完成可视化。将具有预后价值的核心基因作为候选基因，并对其功能进行预测分析。最终共筛选得到207个DEGs，主要富集于细胞外空间；13个核心基因则主要富集于上皮间质转化（epithelial to mesenchymal transition）功能通路。然而在上述候选基因中仅1个可作为有效候选基因。此外，本研究还筛选得到297个与核心基因存在相互作用的miRNA以及349个转录因子（TF）。综上，本研究筛选得到的DEGs、核心基因、miRNA及转录因子，有助于阐明基质矿化影响下骨肉瘤的潜在发病机制，并可为后续研究提供潜在的分子靶标。