Exon-array profiling of peripheral blood cells in the first hours of acute coronary syndrome patients

Identifying novel candidate biomarker gene differentially expressed in the peripheral blood cells of patients with early stage acute myocardial infarction using microarray as a high throughput screening technology. In this study, we used exon array profiling to investigate biomarker gene analyzing 49 patients samples (six time points) and 6 control samples. Whole-blood cell drawn from an antecubital vein at six time points following STEMI diagnosis. The first time (T0) was on patient admission to the Emergency Unit prior to receiving any medication. The second time (T2) blood collection occurred after successful mechanical reperfusion and stenting of the infarct artery within 2 h after T0. Collections for the subsequent four time points were at 12 h (T12), 24 h (T24), 36 h (T36) and 48 h (T48). For the control group, peripheral blood was collected at one time point.

本研究以微阵列（microarray）作为高通量筛选技术，旨在识别早期急性心肌梗死患者外周血细胞中差异表达的新型候选生物标志物基因。本研究采用外显子芯片表达谱分析（exon array profiling）技术，对49例患者样本（含6个时间点）与6例对照样本进行分析。患者样本采集自ST段抬高型心肌梗死（STEMI）确诊后的6个时间点的肘前静脉全血细胞：首个时间点（T0）为患者进入急诊室且未接受任何药物治疗之时；第二个时间点（T2）为T0后2小时内，梗死动脉成功实施机械再灌注并置入支架后采集的血样；后续四个采集时间点分别为T0后12小时（T12）、24小时（T24）、36小时（T36）及48小时（T48）。对照组仅单次采集外周静脉血样。