Supplemental Material for Brenner et al., 2019

Figure S1 shows an overview of mitochondrial RNA editing sites in three Populus species, complementing Figure 1 in the main text.Figure S2 shows RNA editing sites in all affected mitochindrial genes in the four Populus species investigated, complementing Figure 2 in the main text.Figure S3 shows an annotated graphical representation of the mitochondrial genome of Populus alba.Figure S4 shows a multiple sequence alignment of the mitochondrial nad6 gene of different Populus species and accessions, complementing Figure 4 in the main text.File S1 contains the R script used to investigate and graphically display RNA editing.File S2 contains a modified version of Varianttools (Schroeder et al., 2016) optimized for investigating RNA editing.File S3 contains the reference sequences of all mitochondrial genes investigated with regards to RNA editing in this study.Table S1 lists information about the RNA-Seq runs unsed in this study.Table S2 lists the settings of the tools of the CLC Workbench Version 11.0 used for mapping RNA-Seq runs to reference sequences in order to identify potential RNA editing sites.Table S3 contains evaluation tables of potential RNA editing sites.补充图S1展示了3个杨属（Populus）物种的线粒体RNA编辑位点概况，作为对正文图1的补充。补充图S2展示了本次研究涉及的4个杨属物种中所有受影响线粒体基因的RNA编辑位点，作为对正文图2的补充。补充图S3展示了银白杨（Populus alba）线粒体基因组的带注释图形化展示结果。补充图S4展示了不同杨属物种及种质的线粒体nad6基因的多序列比对结果，作为对正文图4的补充。补充文件S1包含了用于开展RNA编辑研究并进行可视化展示的R脚本。补充文件S2包含了经优化以用于RNA编辑研究的改良版Varianttools（Schroeder等，2016）。补充文件S3包含了本研究中所有涉及RNA编辑分析的线粒体基因的参考序列。补充表S1列出了本研究中使用的RNA测序（RNA-Seq）运行样本的相关信息。补充表S2列出了用于将RNA-Seq测序数据比对至参考序列以识别潜在RNA编辑位点的CLC Workbench 11.0版工具的参数设置。补充表S3包含了潜在RNA编辑位点的评估表格。