Diverse MicroRNAs-mRNA networks regulate the priming phase of mouse liver regeneration and of direct hyperplasia

Adult hepatocytes are quiescent cells, that can be induced to proliferate in response to a reduction in liver mass (liver regeneration) or by agents endowed with mitogenic potency (primary hyperplasia). The latter condition is characterized by a more rapid entry of hepatocytes into the cell cycle but the mechanisms responsible for the accelerated entry into the S phase are unknown. Illumina microarray was used to profile mRNA expression in CD-1 mice livers 1, 3 and 6 hours after 2/3 partial hepatectomy (PH) or a single dose of TCPOBOP, a ligand of the constitutive androstane receptor. Ingenuity pathway and DAVID analyses were performed to identify deregulated pathways Illumina microarray were used to profile mRNA expression in CD-1 mice livers 1, 3 and 6 hours after 2/3 partial hepatectomy (PH) or a single dose of TCPOBOP, a ligand of the constitutive androstane receptor. 21 samples in total (TCPOBOP + PH). 3 replicates at each time point: CO, 1,3, and 6 hour

成年肝细胞为静息细胞，可因肝体积缩减（肝再生）或受具有促有丝分裂活性的试剂诱导而发生增殖，该过程即为原发性增生。其中原发性增生的特征为肝细胞更快进入细胞周期，但驱动其加速迈入S期的分子机制尚未阐明。本研究采用Illumina微阵列（Illumina microarray）技术，对经2/3部分肝切除术（partial hepatectomy, PH）处理，或单次给予组成型雄烷受体（constitutive androstane receptor）配体TCPOBOP的CD-1小鼠肝脏，在处理后1、3、6小时的mRNA表达谱进行检测。随后通过Ingenuity通路分析（Ingenuity pathway analysis）与DAVID分析，筛选鉴定失调的信号通路。本数据集共计21个样本（涵盖TCPOBOP处理组与部分肝切除术处理组），各时间点（对照组CO、1小时、3小时及6小时）均设置3次生物学重复。