Humans PBMCs: Balanced constitution vs. Phlegm-dampness constitution

We investigated the expression profiles of lncRNAs and mRNAs in peripheral blood mononuclear cells (PBMCs) from subjects with 13 Phlegm-dampness constitution (PDC) and nine balanced Balanced constitution (BC). The differentially expressed lncRNAs (DElncRNAs) and mRNAs (DEmRNAs) were identified by the “limma” method. Goal was to determine whether long non-coding RNAs (lncRNAs) play a regulatory role in metabolic disease in subjects with PDC. Two-condition experiment, BC vs. PDC. Peripheral blood mononuclear cells (PBMCs). Biological replicates:9 subjects with Balanced constitution (BC), 13 subjects with Phlegm-dampness constitution samples (PDC). This experiment was performed technically as a dual channel. Cy3 is used to the label of the PDC and the BC. Besides, RNA from all samples was as reference_RNA. And Cy5 is used to label the reference_RNA in each chip. Normalized log2 ratio (Cy3/Cy5) representing test/reference.本研究对13例痰湿体质（Phlegm-dampness constitution, PDC）受试者与9例平和体质（Balanced constitution, BC）受试者的外周血单个核细胞（peripheral blood mononuclear cells, PBMCs）中长链非编码RNA（long non-coding RNAs, lncRNAs）和信使RNA（messenger RNA, mRNAs）的表达谱展开分析。采用"limma"方法筛选得到差异表达lncRNAs（DElncRNAs）与差异表达mRNAs（DEmRNAs），旨在明确长链非编码RNA是否在痰湿体质受试者的代谢疾病中发挥调控作用。本实验为双组对照实验，以平和体质组与痰湿体质组为对照，实验样本为外周血单个核细胞。生物学重复设置为：平和体质受试者9例，痰湿体质受试者13例。本实验采用双通道技术开展：Cy3用于标记PDC组与BC组样本，所有样本的RNA均作为reference_RNA，且每张芯片中的reference_RNA均通过Cy5进行标记。最终以归一化后的log2比值（Cy3/Cy5）代表检测样本与参考样本的表达差异。